Randomized Genetically Modified Monoculture Degradation by Aspergillus niger and Penicillium chrysogenum on Varying Concentration of Spent Engine Oil

Abstract

The deleterious impact of spent engine oil on the environment has long been a cause for concern, given its persistent presence and harmful effects on ecosystems. In this study, we assessed the potential of genetically modified or engineered fungi (GMF) in the biodegradation of spent oil contaminated soil. Inoculation of Aspergillus niger (F1) and Penicillium chrysogenum (F2) on spent oil-contaminated soil samples at various concentrations (1%, and 5%) over a 28-day period was conducted. The optical density (OD) values of the various samples were analyzed over the observation period. Both F1 and F2 at 1% and 5% concentrations exhibited a significant decrease in OD values, indicating effective degradation of the spent oil. However, F1 showed a slower and more pronounced OD reduction compared to F2. Interestingly, F2 demonstrated a faster and more prominent reduction in optical density values, particularly during the later stages of the experiment, highlighting potentially superior efficacy compared with F1. These findings highlight the promise of these fungal isolates as viable candidates for bioremediation agents, showcasing their ability to contribute to environmentally friendly and sustainable solutions for hydrocarbon-rich pollutant cleanup.