Isolation of Mesenchymal Cells and their Relationship with Blood Vessels Formation at Early Stages of Gestation in the Human Fetus

Abstract

Objective: The aim of this study was to compare the areas occupied by  mesenchymal cells and the areas of blood vessels for the same stage of gestation. In the same way, the lengths and widths of the mesenchymal cells grouped around the vascularization and the same isolated cells were measured and compared. Materials and method: Tissue of the temporal region of 10 aborted human fetuses at 12 weeks of gestation was removed in 10 cubic millimeter blocks. The blocks were embedded in paraffin, and these paraffin-embedded blocks were sectioned serially in sagittal planes 6 μm wide with a rotatory microtome. Hematoxylin-eosin staining was used in each of the histological sections for detect or not the presence of mesenchymal cells and vascularization respectively. The area occupied by blood vessels and mesenchymal cells was measured and compared with the use of image j software. The student's T-test was used to compare the areas occupied by  mesenchymal cells and the areas of vascularization. Similarly, the same statistical test was used to compare the lengths and width between cells that occupied colonies or were isolated. Conclusion: mesenchymal cells occupied a larger area than blood vessels zones. The width of the isolated cells was considerably larger. However, the comparison of cell lengths between isolated cells and those occupying colonies did not show marked differences. The isolated cells were observed close to areas of osteoid tissue.